Equipment:
![[NIKON E400]](/imgs/path/nikon_E400.jpg "Nikon Eclipse E400")
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- Upright Nikon Eclipse E400 equipped with a color CCD camera (Insight)
and Spot software, 4, 10, 40, 60 and 100x objectives for still digital imaging
of stained histology preparations.
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![[Axiovert 100]](/imgs/path/axiovert_100.jpg "Axiovert 100TV")
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- Inverted Axiovert 100TV Zeiss microscope equipped for fluorescence with filter cubes
for FITC/GFP, Rhodamine/Cy3 and Cy5.5 and 10, 20 (phase), 40 (phase), 60 and 100x objectives.
Equipped with a low-light video rate camera for live time digital imaging under
transillumination/phase contrast and a Photometrics CoolSnap HQ CCD camera for still digital
fluorescence images, both coupled to a G4 Macintosh computer running IP Labs software and ImageJ.
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![[Nikon 80i]](/imgs/path/nikon_80i.jpg "Nikon 80i")
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- Upright Nikon 80i epifluorescence microscope equipped with 10x (phase), 20x, 40x and 60x
Plan Fluor short working distance objectives, and the following filter cubes.
- #96300 UV-1A DAPI filter set: Excitation 360-370, Dichroic Mirror DM380, Emission LP420
- #96320 FITC/GFP HyQ filter set: Excitation 460-500, Dichroic Mirror DM505, Emission 510-560
- #96305 G-1B filter set: Excitation 541-551, Dichroic Mirror DM565, Emission LP590
- #41023 Cy5.5 filter set: Excitation 627-672, Dichroic Mirror DM685, Emission 685-735
- #41009 Cy7/AF750 filter set: Excitation 673-748, Dichroic Mirror DM750, Emission 765-835
Equipped with a Photometrics Cascade low intensity back-illuminated CCD camera coupled
to a G4 Macintosh computer running IP Labs software. Live focus capability under both transillumination
and fluorescence with 100 msec refresh rate.
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![[Nikon TE2000]](/imgs/path/nikon_TE2000.jpg "Leica")
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- Inverted Nikon TE2000 epifluorescence microscope equipped with 4, 10, 20 and 40x phase/fluorescence
objectives and GFP/FITC, Rhodamine/Cy3, Cy5, Cy5.5 and Cy7 filter cubes. Equipped with a color Insight
CCD camera and Spot Software for digital imaging of live cells.
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![[Nikon Eclipse 50i]](/imgs/path/nikon_50i.jpg "Nikon Eclipse 50i")
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- Upright Nikon Eclipse 50i Light/Fluorescence equipped with a
color CCD camera (Insight) and Spot software (4.1) 4, 10, 20,
40 and 100x objectives for still digital imaging of stained
histology preparations.
- Upright Nikon Eclipse 50i Light microscope equipped with 4,
10, 20, 40 and 100x objectives for imaging of stained
histology preparations.
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![[Leica Cryotome CM1900]](/imgs/path/cryotome.jpg "Leica Cryotome CM1900")
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- Leica (CM1900) Rapid Sectioning Cryostat.
- Two separate cooling systems; cryochamber down to -35°C and specimen temperature down to -50°C.
- Section thickness settings from 1 to 60um.
- 90° prism head mounts on the specimen head for quick freezing specimens in place.
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![[Leica Cryotome CM1900]](/imgs/path/mac.gif "Leica Cryotome CM1900")
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