FRI imaging facility

The overall goal of this facility is provide imaging equipment for fluorescent reflectance optical imaging. A number of different multichannel imaging systems are available (for OV100 click here), depending upon the imaging needs.

Olympus OV100

The Olympus OV100 system is a hybrid system of a planar reflectance fluorescent imaging system, and a high-power microscope system. Filters available for this system include green, red, far red and near infrared. Images can be captured of whole mice, and the high magnification zoom lens allows for detailed imaging of specific region of interest. This system is equipped with isoflurane anesthesia and has a heating plate to meintain subject body temperature throughout the course of imaging.

Ov100

Siemens BonSAI

The Siemens bonSAI system is a fluorescence optical scanner for in vivo imaging of small animals. Consisting of a light source, CCD camera, and light-tight acquisition chamber, it allows for the imaging of one animal at a time and was designed for rapid imaging and ease of use. Filters are available for imaging green ((l max = 447 nm excitation/527 nm emission), red (527 nm excitation/610 nm emission), far red (660 nm excitation/735 nm emission), and near infrared (762 nm excitation/800 nm emission).

Bonsai

CRi Maestro

The CRi Maestro system is designed for multispectral fluorescence-based small animal imaging and analysis in vivo. Included in this system is a light source, CCD camera, and light-tight imaging chamber. The system software allows the elimination of autofluorescence in the desired spectral range, thus improving sensitivity and quantitative accuracy. Excitation/emission filter sets are available for imaging blue (l max = 465 nm excitation/515 nm emission), green (529 nm excitation/580 nm emission), yellow (590 nm exitation/645 nm emission), red (649 nm excitation/700 emission), and far red (690 nm excitation/750 nm emission) fluorophores.

Maestro

Publications

  1. Gee MS, Upadhyay R, Bergquist H, Weissleder R, Josephson L, Mahmood U Multiparameter noninvasive assessment of treatment susceptibility, drug target inhibition and tumor response guides cancer treatment. Int J Cancer. 2007; :ePub.
  2. Tam JM, Upadhyay R, Pittet MJ, Weissleder R, Mahmood U Improved in vivo whole-animal detection limits of green fluorescent protein-expressing tumor lines by spectral fluorescence imaging. Mol Imaging. 2007;6(4):269-76.
  3. Ntziachristos V, Ripoll J, Wang L, Weissleder R. Looking and Listening to light: the evolution of whole-body photonic imaging. Nat Biotechnol. 2005;23:313-320.
  4. Chen J, Tung CH, Allport JR, Chen S, Weissleder R, Huang PL. Near-Infrared Fluorescent Imaging of Matrix Metalloproteinase Activity After Myocardial Infarction. Circulation. 2005;111:1800-1805.
  5. Figueiredo JL, Alencar H, Weissleder R, Mahmood U. Near infrared thoracoscopy of tumoral protease activity for improved detection of peripheral lung cancer. Int J Cancer. 2005;118(11):2672-7.
  6. Alencar H, King R, Funovics M, Stout C, Weissleder R, Mahmood U. A novel mouse model for segmental orthotopic colon cancer. Int J Cancer. 2005;117(3):335-9
  7. Weissleder, R. and Ntziachristos, V. Shedding light onto live molecular targets. Nat Med. 2003;9:123-8.