Bioluminescence imaging (BLI)

The overall goal of this facility is provide in vivo bioluminescence imaging capabilities as well as software support for image quantitation, fusion, and display.

CMIR homebuilt system

The bioluminescence imaging system has been designed and assembled at CMIR. It consists of a liquid nitrogen-cooled, photon-counting Roper Scientific CCD in a light tight box, with a large aperture zoom lens for whole animal imaging. The system can operate in single wavelength mode, for typical luciferase experiments, or has (with slightly lower sensitivity) multiwavelength capabilities for near simultaneous imaging of different luminescent constructs.

IVIS system

The CMIR Xenogen IVIS Imaging System 100 consists of a back-illuminated CCD with a closed-cycle refrigeration system, a camera controller, and a light-tight box for bioluminescence imaging. The field of view can be adjusted from 10-25 cm to allow the imaging of two to five mice at a time on a heated platform.