Bioluminescence imaging (BLI)
The overall goal of this facility is provide in vivo bioluminescence imaging capabilities as well as software support for image quantitation, fusion, and display.
Center for Systems Biology (CSB) in-house built system
The BLI system has been designed and assembled at the Center for Systems Biology (previously the CMIR). It consists of a liquid nitrogen-cooled, photon-counting Roper Scientific CCD contained within a light-tight box, and a large aperture zoom lens for whole animal imaging. The system can operate in single wavelength mode (appropriate for typical luciferase experiments), or has (with slightly lower sensitivity) multiwavelength capabilities for near simultaneous imaging of different luminescent constructs.
The CSB Xenogen IVIS Imaging System 100 consists of a back-illuminated CCD camera with a closed-cycle refrigeration system, a camera controller, and a light-tight box for bioluminescence imaging. The field of view can be adjusted from 10-25 cm to allow for imaging of two to five mice at a time on a heated platform.