Bioluminescence imaging (BLI)

The overall goal of this facility is provide in vivo bioluminescence imaging capabilities as well as software support for image quantitation, fusion, and display.

CMIR homebuilt system

The bioluminescence imaging system has been designed and assembled at CMIR. It consists of a liquid nitrogen-cooled, photon-counting Roper Scientific CCD in a light tight box, with a large aperture zoom lens for whole animal imaging. The system can operate in single wavelength mode, for typical luciferase experiments, or has (with slightly lower sensitivity) multiwavelength capabilities for near simultaneous imaging of different luminescent constructs.

Bli1 Bli2

IVIS system

The CMIR Xenogen IVIS Imaging System 100 consists of a back-illuminated CCD with a closed-cycle refrigeration system, a camera controller, and a light-tight box for bioluminescence imaging. The field of view can be adjusted from 10-25 cm to allow the imaging of two to five mice at a time on a heated platform.

Xeno2

Publications

  1. Kock N, Kasmieh R, Weissleder R, Shah K Tumor Therapy Mediated by Lentiviral Expression of shBcl-2 and S-TRAIL. Neoplasia. 2007;9(5):435-42.
  2. Shah K, Bureau E, Kim DE, Yang K, Tang Y, Weissleder R, Breakefield XO. Glioma therapy and real-time imaging of neural precursor cell migration and tumor regression. Ann Neurol. 2005;57(1):34-41.
  3. Tang Y, Kim M, Carrasco D, Kung AL, Chin L, Weissleder R. In vivo assessment of RAS-dependent maintenance of tumor angiogenesis by real-time magnetic resonance imaging. Cancer Research. 2005;65(18):8324-30.
  4. Shah K, Tung CH, Breakefield X, Weissleder R. In vivo imaging of S-TRAIL-mediated tumor regression and apoptosis. Molecular Therapy. 2005;11 (6):926-931.
  5. Tannous BA, Kim DE, Fernandez JL, Weissleder R, Breakefield XO. Codon-optimized gaussia luciferase cDNA for Mammalian gene expression in culture and in vivo. Molecular Therapy. 2005;11(3):435-43.
  6. Shah, K., Tung, C. H., Chang, C. H., Slootweg, E., OLoughlin, T., Breakefield, X. O., and Weissleder, R. In vivo imaging of HIV protease activity in amplicon vector-transduced gliomas. Cancer Research. 2004;64:273-8.
  7. Shah, K., Tang, Y., Breakefield, X., and Weissleder, R. Real-time imaging of TRAIL-induced apoptosis of glioma tumors in vivo. Oncogene. 2003;22:6865-72.
  8. Tang, Y., Shah, K., Messerli, S. M., Snyder, E., Breakefield, X., and Weissleder, R. In vivo tracking of neural progenitor cell migration to glioblastomas. Hum Gene Ther. 2003;14:1247-54.