Normalized Born
Fluorescence reconstructions are enabled via the use of a normalized data set. In particular we have found that ratios of fluorescence over excitation wavelength measurements give robust reconstruction performance that is insensitive to several experimental uncertainties and to tissue optical heterogeneity. Figures 1 and 2 show early results of the ability of the system to image two fluorescent tubes immersed in diffuse media.
Figure. 1
Fluorescence reconstruction of the 1st generation FMT system with three layers along z.
The fluorescence measurements are normalized by use of Uinc, i.e., the excitation field collected when the two absorbing
fluorescent objects were no immersed.
Figure. 2
Fluorescence reconstruction of the 1st generation FMT
system with three layers along z. The fluorescence measurements are normalized by use of Uinc0,
i.e., the excitation field collected with the two absorbing fluorescent objects immersed.
For details see Ntziachristos V, et.al. Opt. Lett. 26(12): 893-895 (2001)