The CMIR Flow Cytometry Core provides orientation and training in FACS analysis (profiling) on a BD FACSCalibur for individuals who want to analyze samples containing 1 to 4 compatible fluorochromes. This includes training or consultation in staining techniques and sample preparation and orientation and training in sample acquisition and data analysis using the BD Biosciences proprietary software, CellQuest.

Flow cytometry describes the analysis of light diffraction and fluorescent properties of a population of cells or particles as they pass through one or more lasers. It is a powerful technique as it provides real quantitative information on a cell by cell or particle by particle basis.

1. Size and granularity (internal complexity) of the cell
2. The cell surface expression of particular proteins using antibodies and fluorescent reporters
3. Intracellular protein levels e.g. chemokines
4. DNA content and cell cycle analysis
5. Cellular uptake of particular fluorescent agents including CLIO-based particles
6. Cell proliferation using cytoplasmic dyes (CFSE etc)

1. Becton Dickinson FACScalibur equipped with both 4-line Argon and HeNe lasers for four color profiling and analysis
2. Access to 2 other BD FACScalibur instruments and a separate analysis station in the Center for Aids Research (5th floor)
3. Access to a BD LSR II for up to 11 color profiling, analysis and sorting in the Center for Aids Research

• FACS resources on CMIR Intranet (password required)
• General FACS Regulations
• Training
• FACS Service